Effects of Chloramine-T And CuSO4 On Enzyme Activity Of Glucose 6-Phosphate Dehydrogenase From Rainbow Trout (Oncorhynchus Myskiss) Erythrocytes In Vitro An In Vivo

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dc.contributor.author Çiltas, Abdulkadir
dc.contributor.author Erdogan, Orhan
dc.contributor.author Hisar, Olcay
dc.contributor.author Çiftçi, Mehmet
dc.date.accessioned 2012-01-19T20:10:41Z
dc.date.available 2012-01-19T20:10:41Z
dc.date.issued 2003
dc.description.abstract Traditional treatments of parasitic and bacterial diseases are based on chemotherapeutic com- pounds such as chloramine-T and CuSO4. Although many compounds are used in fish treat- ments, their undesirable effects are not known. In this study, glucose 6-phosphate dehydroge- nase (G6PD) was purified from rainbow trout (Oncorhynchus mykiss) erythrocytes by hemolysate preparation, ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel chromatography in a single day. The enzyme, with a specific activity of 14.51 EU/mg protein, was purified 906.8-fold with a yield of 70.38%. To check the purity of the enzyme, SDS poly- acrylamide gel electrophoresis was performed, which showed a single band. The effects of chlo- ramine-T and copper sulfate (CuSO4) on the G6PD were investigated in vitro. Chloramine-T and CuSO4 had inhibitory effects on the enzyme. I50 values of the chemotherapeutic compounds were determined by plotting percent activity and Ki values, and types of inhibition were deter- mined for each compound by means of Lineweaver-Burk graphs. In vivo studies showed that G6PD in rainbow trout erythrocytes was significantly inhibited by CuSO4 in one hour but not inhibited by chloramine-T.
dc.format.extent 10 pages
dc.identifier.citation Çiltas, A., Erdogan, O., Hisar, O., & Çiftçi, M. (2003). Effects of Chloramine-T And CuSO4 On Enzyme Activity Of Glucose 6-Phosphate Dehydrogenase From Rainbow Trout (Oncorhynchus Myskiss) Erythrocytes In Vitro An In Vivo. The Israeli Journal of Aquaculture - Bamidgeh, 55(3), 187-196.
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/19079
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh
dc.subject chloramine-T, CuSO4, G6PD, inhibition, Oncorhynchus mykiss
dc.subject.lcsh Fish culture--Israel--Periodicals.
dc.subject.lcsh Fish culture--Periodicals.
dc.subject.lcsh Aquaculture--Israel--Periodicals.
dc.subject.lcsh Aquaculture--Periodicals.
dc.title Effects of Chloramine-T And CuSO4 On Enzyme Activity Of Glucose 6-Phosphate Dehydrogenase From Rainbow Trout (Oncorhynchus Myskiss) Erythrocytes In Vitro An In Vivo
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IJA Volume 55, Issue 3, 2003