Preliminary Study on Hybridization of Brown Trout (Salmo trutta macrostigma) and Rainbow Trout (Oncorhynchus mykiss) Using Cryopreserved Sperm

Abstract

This study examined the effect of two different extenders (carbohydrate and mineral based), supplemented with 10% Dimethyl sulfoxide (DMSO), on cryopreservation of brown trout (Salmo trutta macrostigma) sperm used for hybridization with rainbow trout (Oncorhunchus mykiss). The carbohydrate based extender resulted in higher fertilization (42.5±1.4%) than the mineral based extender and there was no difference in fertilization rates (p>0.05). This study successfully demonstrated that it is possible to produce Salmo trutta macrostigma x Oncorhynchus mykiss hybrids using cryopreserved sperm from brown trout.