Molecular Cloning and Response to Water Temperature and Nutrient Manipulation of Insulin-like Growth Factor (IGF) Genes in Golden Pompano Trachinotus ovatus (Linnaeus 1758) Larvae
Molecular Cloning and Response to Water Temperature and Nutrient Manipulation of Insulin-like Growth Factor (IGF) Genes in Golden Pompano Trachinotus ovatus (Linnaeus 1758) Larvae
Date
2016
Authors
Zhenhua Ma
Jing Hu
Yajuan Liu
Rui Yang
Jian G. Qin
Dianrong Sun
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
In this study, insulin-like growth factor I (IGF I) and IGF II in golden pompano larvae were cloned and analyzed. In the first trial, IGF expression during ontogeny of larvae in the first 18-days of their life was explored, and then the response of IGFs to water temperature (23, 26, and 29oC) on 12 day post hatching (DPH) and 18 DPH were compared. On 28 DPH, the response of IGFs to the manipulation of nutrients was evaluated. The expression of IGF I increased with the increase of fish age, and was not significantly affected by water temperature. The expression of IGF II was affected by water temperature on 12 DPH and 18 DPH. The expression of IGF II at 23oC was significantly higher than at 26oC and 29oC. The expression of IGFs in fish larvae on 28 DPH was not concomitant with nutrient manipulation. This study detected the gene expression of IGFs at the early stage of golden pompano larvae. The time dependent expression of IGF genes in fish larvae is important to understand the ontogenetic development and growth of fish larvae in early life.
Description
Keywords
Insulin-like growth factors,
gene expression,
temperature,
enrichment,
golden pompano,
Trachinotus ovatus,
Fish culture--Israel.,
Fish culture.
Citation
Extent
13 pages
Format
Geographic Location
Time Period
Related To
The Israeli Journal of Aquaculture - Bamidgeh
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.