Ontogeny of Nile tilapia (Oreochromis niloticus) Immunoglobulin Type M Antibody Response

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dc.contributor.authorKuendee, Natthaporn
dc.contributor.authorKlaynongsruang, Sompong
dc.contributor.authorBunyatratchata, Wandee
dc.contributor.authorTengjaroenkul, Bundit
dc.contributor.authorNgamcharoen, Kittipoj
dc.contributor.authorDaduang, Jureerut
dc.contributor.authorUngarreevittaya, Piti
dc.contributor.authorDaduang, Sakda
dc.date.accessioned2016-05-05T22:57:41Z
dc.date.available2016-05-05T22:57:41Z
dc.date.issued2015
dc.description.abstractNile tilapia (Oreochromis niloticus, Cichlidae) are cultured worldwide, however, the study of humoral immunity in these fish has been neglected, leading to mismanagement of prevention of common diseases by vaccination. In this study we purified and characterized the Nile tilapia immunoglobulin type M (IgM). In addition, we have described the production of a mouse polyclonal antibody for the investigation of the onset of antibody responses. After one-step purification using protein G sepharose beads, SDSPAGE, and mass fingerprint analysis we found that the heavy chain of Nile tilapia IgM was 70 kDa, whereas the light chain was 27 kDa. Western immunoblotting techniques using mouse anti-Nile tilapia IgM antibody, produced by intraperitoneal injection with purified Nile tilapia IgM for 3, periods with booster inoculations every 10 days, could effectively detect the onset of antibody responses in Nile tilapia sera at 42 days post-hatch.
dc.format.extent8 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/49215
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectantibody ontogeny
dc.subjectantibody production
dc.subjectWestern immunoblotting
dc.subjectimmunoglobulin M
dc.subjectNile tilapia
dc.subjectOreochromis niloticus
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture.
dc.titleOntogeny of Nile tilapia (Oreochromis niloticus) Immunoglobulin Type M Antibody Response
dc.typeArticle
dc.type.dcmiText

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Volume 67, 2015