Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.
Date
2007
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Israeli Journal of Aquaculture - BAMIGDEH
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Abstract
Genetic differences between Capoeta capoeta capoeta, Capoeta capoeta umbla, and Capoeta tinca were determined using PCR-RFLP of mtDNA cytochrome-b (Cyt-b) by amplifying approxi- mately 400-500 bp of this region from each of the three subspecies. The restriction enzymes SpeI and HinfI did not indicate genetic differences but AluI and HpaII did. Thus, PCR-RFLP of the mtDNA was used to distinguish between closely related subspecies without having to ana- lyze the entire DNA sequence of specimens. Use of this technique demonstrated that the Cyt-b regions of the three subspecies have different base sequences. The similarity between C. c. capoeta and C. c. umbla is 71.4% while C. tinca is more distant (50% for C. c. umbla and 33.3% for C. c. capoeta). The Cyt-b similarity is consistent with morphological and taxonomical similar- ities. PCR-RFLP can serve as a tool for genetically identifying subspecies of fish in nature and in aquaculture.
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Capoeta spp., Cytochrome-b, MtDNA, PCR, RFLP, Fish culture--Israel--Periodicals., Fish culture--Periodicals., Aquaculture--Israel--Periodicals., Aquaculture--Periodicals.
Citation
Aksakai, E., & Erdogan, O. (2007). Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.. The Israeli Journal of Aquaculture - Bamidgeh, 59(4), 206-211.
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6 pages
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