Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.
| dc.contributor.author | Aksakal, Ecrument | |
| dc.contributor.author | Erdogan, Orhan | |
| dc.date.accessioned | 2012-01-19T20:21:27Z | |
| dc.date.available | 2012-01-19T20:21:27Z | |
| dc.date.issued | 2007 | |
| dc.description.abstract | Genetic differences between Capoeta capoeta capoeta, Capoeta capoeta umbla, and Capoeta tinca were determined using PCR-RFLP of mtDNA cytochrome-b (Cyt-b) by amplifying approxi- mately 400-500 bp of this region from each of the three subspecies. The restriction enzymes SpeI and HinfI did not indicate genetic differences but AluI and HpaII did. Thus, PCR-RFLP of the mtDNA was used to distinguish between closely related subspecies without having to ana- lyze the entire DNA sequence of specimens. Use of this technique demonstrated that the Cyt-b regions of the three subspecies have different base sequences. The similarity between C. c. capoeta and C. c. umbla is 71.4% while C. tinca is more distant (50% for C. c. umbla and 33.3% for C. c. capoeta). The Cyt-b similarity is consistent with morphological and taxonomical similar- ities. PCR-RFLP can serve as a tool for genetically identifying subspecies of fish in nature and in aquaculture. | |
| dc.format.extent | 6 pages | |
| dc.identifier.citation | Aksakai, E., & Erdogan, O. (2007). Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp.. The Israeli Journal of Aquaculture - Bamidgeh, 59(4), 206-211. | |
| dc.identifier.issn | 0792-156X | |
| dc.identifier.uri | http://hdl.handle.net/10524/19228 | |
| dc.publisher | Israeli Journal of Aquaculture - BAMIGDEH | |
| dc.relation.ispartofseries | The Israeli Journal of Aquaculture - Bamidgeh | |
| dc.subject | Capoeta spp., Cytochrome-b, MtDNA, PCR, RFLP | |
| dc.subject.lcsh | Fish culture--Israel--Periodicals. | |
| dc.subject.lcsh | Fish culture--Periodicals. | |
| dc.subject.lcsh | Aquaculture--Israel--Periodicals. | |
| dc.subject.lcsh | Aquaculture--Periodicals. | |
| dc.title | Use of PCR-RFLP Analysis of mtDNA Cytochrome-b Gene to Determine Genetic Differences in Capoeta spp. |
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