A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems

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dc.contributor.authorCecchini, Stefano
dc.contributor.authorRocchina Caputo, Anna
dc.date.accessioned2012-03-02T22:08:55Z
dc.date.available2012-03-02T22:08:55Z
dc.date.issued2012
dc.description.abstractThis paper describes a simple spectrophotometric procedure for determining NO3--N in water samples. In the proposed method, NO2--N of a water sample is first removed from the water with sulfamic acid. Then, NO3--N is reduced to NO2--N using vanadium (III) chloride and a detection reagent is added. When rapid collection of data is necessary, this method can be adapted to a microtechnique using a 96-well microplate, resulting in a detection limit of 5 NO3--N mg/l. Using a larger volume spectrophotometer cell (5 cm) lowers the detection limit to 0.5 NO3--N mg/l. The proposed method is applicable for routine analysis of water in aquaculture systems and wastewaters in which medium-high NO3--N concentrations are expected. Benefits of the method are reduction of the laborious preparation and avoidance of contact with the harmful substances typical of the cadmium reduction method.
dc.format.extent6 pages
dc.identifier.citationStefano Cecchini, Anna Rocchina Caputo. (2012). A Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems. The Israeli Journal of Aquaculture - Bamidgeh, 64, 6 pp.
dc.identifier.issn0792-156X
dc.identifier.otherIJA_64.2012.698
dc.identifier.urihttp://hdl.handle.net/10524/22921
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleA Direct Spectrophotometric Assay for Evaluating Nitrate-Nitrogen in Intensive Aquaculture Systems

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Volume 64, 2012