The use of supplementary feed with ethanol extract of <em>Syzygium nervosum</em> leaf to prevent acute hepancreatic necrosis (AHPND) in white shrimp (<em>Litopenaeus vannamei</em>)
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2025
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77
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This study evaluated the effects of supplementing shrimp diets with an ethanol extract of Syzygium nervosum leaf on growth performance, survival rate, immune parameters, and resistance to AHPND in white shrimp (L. vannamei). Shrimp juveniles (weighing 1.05 ± 0.04 g) were fed diets supplemented with varying concentrations of S. nervosum leaf extract, including 12.5 g/kg (NT1), 25.0 g/kg (NT2), 37.5 g/kg (NT3), and a control diet without S. nervosum leaf extract (DC). After 28 days of feeding, the shrimp were challenged with Vibrio parahaemolyticus. The results showed no significant differences in growth performance and feed conversion ratio between the experimental groups and the control group (p>0.05). However, the survival rates of shrimp in the supplemented groups (93.4-94.1%) were significantly higher than those in the control group (90.0%) (p<0.05). Furthermore, shrimp in the experimental groups exhibited higher total haemocyte counts, phenoloxidase activity, and lysozyme activity than the control (p<0.05). After 14 days of being challenged with V. parahaemolyticus, the cumulative mortality of shrimp in the supplemented groups was significantly lower than that of the control group (p<0.05). The study recommends supplementing shrimp diets with S. nervosum leaf extract at a concentration of 12.5 g/kg as the optimal level for practical application in white shrimp farming.
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Acute hepatopancreatic necrosis disease, Growth performance, immune response, Litopenaeus vannamei, Vibri parahaemolyticus, Syzygium nervosum leaf extract
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The Israeli Journal of Aquaculture - Bamidgeh
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