Volume 77, Issue 1, 2025

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https://hdl.handle.net/10524/81137

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    Dietary incorporation of Bacillus subtilis, enhances growth performance, survival rate, gametogenesis and intestinal health, in zebrafish (Danio rerio)
    (2025) Dede, Kemal; Cek, Sehriban
    This study assessed the probiotic effects of dietary supplementation with Bacillus subtilis HBB 493® on growth, survival, gametogenesis, and intestinal health of Zebrafish (Danio rerio). 600 juveniles were divided into five experimental groups namely: Control group I (0.0 cfu/g), group II (6.5x109 cfu/g), group III (1.3x1010 cfu/g), group IV (2.6x1010 cfu/g) and group V(3.9x1010 cfu/g). Each treatment and control had three replicates, while each replicate had 40 fish. The duration of the experiment was 100 days. At the termination of the experiment, gonads, and intestine were histologically assessed. Growth parameters, in the fish fed 3.9x10^10^ cfu/g were significantly different from those observed in the control, group II, and group III B. subtilis fed groups (P<0.05), and group V was the best. There were no significant differences in the survival rate between treatment groups including control (P>0.05). The histological observations of the gonads reveal differences among the fish fed different levels of B. subtilis. Fish fed diets II, III, IV and V had more developed gonads compared to those on diets without B. subtilis. The status of villus and goblet cells was used to assess the intestinal health of the fish fed diets supplemented with B. subtilis. Villus and goblet cells were intact in all the different levels of B. subtilis supplemented groups. The present study suggested that the dietary supplementation of 3.9x1010 cfu/g probiotic B. subtilis should be used for the enhancement of growth parameters, survival rate, gametogenesis and intestinal health in ornamental zebrafish.
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    Effect of chronic heat stress in roughskin sculpin (Trachidermus fasciatus)
    (2025) Liu, Zitong; San, Lize; He, Zhongwei; Liu, Yufeng; Han, Tian; Gong, Chunguang; Ren, Jiangong; Ren, Yuqin; Hou, Jilun
    The roughskin sculpin (Trachidermus fasciatus) belongs to the family Cottidae and is a small carnivorous catadromous fish distributed along the coasts of China, Korea, and Japan. The effects of heat stress on roughskin sculpin have yet to be systematically performed. This study aimed to explore the effects of chronic heat stress on the roughskin sculpin. The survival rate, gill histopathological changes, and heat stress protein gene expression patterns of roughskin sculpin were analyzed after different temperature stresses of 19 ℃, 22 ℃, 25 ℃, 28 ℃, and 31 ℃ for 0, 3, 6, 9, and 12 days. The results revealed that roughskin sculpin could not survive at 33 ℃. Chronic heat stress can cause severe damage to gill tissue, reducing the gills' respiratory efficiency. Moreover, the expressions of hspb1, hspb7, and hspb11 changed significantly after heat stress. In the 31 ℃ treatment group, as stress time increased, hspb1, hspb7, and hspb11 expression initially increased and then decreased. This study’s results provide a reference for the study of heat stress physiology of roughskin sculpin as well as a new idea and method for evaluating and breeding heat stress traits of roughskin sculpin.
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    Supplementation of Yellow River carp diet with lutein and ferrous fumarate: Growth performance, digestive enzyme activity, skin pigmentation, and intestinal microbiota
    (2025) Ren, Hongtao; Cao, Xiang; Guo, Xiangzhi; Yuan, Peng
    In this study, the effect of the diet with lutein and ferrous fumarate on Yellow River carp (Cyprinus carpio) was studied, aiming to evaluate skin pigmentation, intestinal digestive enzymes, intestinal microbial diversity, and growth performance. Three experimental diets, including a control group, a lutein group (150mg/kg lutein), and a lutein and ferrous fumarate mixture group (150mg/kg lutein and 100mg/kg ferrous fumarate), were designed. The carp (N=135; 25.0±2.0g) were fed with experimental diets for 42 days. The results showed that the intestosomatic index (ISI) and viscerosomatic index (VSI) of the carp fed with lutein and ferrous fumarate were increased, accompanied by significant changes in body color, with the higher value of blue (b), color difference (ΔE) and chroma (Ch) compared with control group (P <0.05). Meanwhile, the higher activity of amylase, lipase, and trypsin were observed in the mixture group (P<0.05). High-throughput sequencing and Venn diagrams revealed that lutein or ferrous fumarate has obvious effects on the intestinal microbiota community of carp. The abundance of Actinobacteria and Flavobacterium was significantly increased in the carp fed with the mixture group compared with the control group. In conclusion, the addition of lutein and ferrous fumarate to the feed can change the skin pigmentation and intestinal microbial composition of Yellow River carp, thereby enhancing the coloring effect and digestive function of the fish. These findings provide valuable insights for optimizing feed formulation and aquaculture management, which can contribute to the improvement of the quality and farming efficiency of Yellow River carp.