In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax
Date
2004
Authors
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Israeli Journal of Aquaculture - BAMIGDEH
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
The melatonin-generating system of the European sea bass, Dicentrarchus labrax, was studied in vitro using a perfusion tissue culture methodology. The examined properties included photic entrainment in the pineal gland, and circadian rhythmicity in alternating dark and light, in contin- uous darkness, and in continuous light. Pineal glands were removed from adult fish and placed in a perfusion tissue culture at a constant temperature of 24°C. Melatonin discharge into the cul- ture medium was measured using radioimmunoassay. Melatonin concentrations during a pho- toperiod of 12h light:12h dark were low during the light periods and high during the dark. The melatonin discharge was inhibited by continuous light but strong and rhythmic in continuous dark with a circadian period (Tau) of about 24 h (n = 10). The results demonstrate that the European sea bass pineal gland is photosensitive, with ability to be entrained, and contains an internal cir- cadian oscillator that regulates melatonin production.
Description
Keywords
circadian oscillator; Dicentrarchus labrax; melatonin; pineal gland, Fish culture--Israel--Periodicals., Fish culture--Periodicals., Aquaculture--Israel--Periodicals., Aquaculture--Periodicals.
Citation
Ron, B. (2004). In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax. The Israeli Journal of Aquaculture - Bamidgeh, 56(4), 281-285.
Extent
5 pages
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.