In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax

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dc.contributor.authorRon, Benny
dc.date.accessioned2012-01-19T20:13:43Z
dc.date.available2012-01-19T20:13:43Z
dc.date.issued2004
dc.description.abstractThe melatonin-generating system of the European sea bass, Dicentrarchus labrax, was studied in vitro using a perfusion tissue culture methodology. The examined properties included photic entrainment in the pineal gland, and circadian rhythmicity in alternating dark and light, in contin- uous darkness, and in continuous light. Pineal glands were removed from adult fish and placed in a perfusion tissue culture at a constant temperature of 24°C. Melatonin discharge into the cul- ture medium was measured using radioimmunoassay. Melatonin concentrations during a pho- toperiod of 12h light:12h dark were low during the light periods and high during the dark. The melatonin discharge was inhibited by continuous light but strong and rhythmic in continuous dark with a circadian period (Tau) of about 24 h (n = 10). The results demonstrate that the European sea bass pineal gland is photosensitive, with ability to be entrained, and contains an internal cir- cadian oscillator that regulates melatonin production.
dc.format.extent5 pages
dc.identifier.citationRon, B. (2004). In Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax. The Israeli Journal of Aquaculture - Bamidgeh, 56(4), 281-285.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19121
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectcircadian oscillator; Dicentrarchus labrax; melatonin; pineal gland
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleIn Vitro Melatonin Rhythm Reveals A Clocked Pineal In The European Sea Bass, Dicentrarchus Labrax

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IJA Volume 56, Issue 4, 2004