Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum

dc.contributor.author Harikrishnan, Ramasamy
dc.contributor.author Kim, Man-Chul
dc.contributor.author Kim, Ju-Sang
dc.contributor.author Balasundaram, Chellam
dc.contributor.author Jawahar, Sundaram
dc.contributor.author Heo, Moon-Soo
dc.date.accessioned 2012-01-19T20:28:01Z
dc.date.available 2012-01-19T20:28:01Z
dc.date.issued 2010
dc.description.abstract The  chemical  constituents  of  decoction  (individual)  and  concoction  (mixed)   of   ethanolic   leaf   extracts   from   Azadirachta   indica   (neem)   and   Ocimum   sanctum   (tulsi)   were   analyzed   by   gas   chromatography-­mass   spectro-­ photometery  (GC-­MS).  Decoctions  of  A.  indica  and  O.  sanctum  had  24  and   33   constituents,   respectively.   Mixed   together,   26   compounds   were   identified.   Four   major   (high   percentage)   compounds   were   identified   in   A.   indica:   n-­hexadecanoic   acid   (14.34%),   phytol   (19.96%),   9,12,15-­octa-­ decatrienoic   acid,   (Z,Z,Z)-­   (18.57%),   and   vitamin   E   (11.37%).   Three   major  compounds  were  identified  in  O.  sanctum:  phenol,2-­methoxy-­3-­(2-­ propenyl)   (15.32%),   9,12,15-­octadecatrienoic   acid,(Z,Z,Z)-­   (16.94%),   and  9,12,15-­octadecatrienoic  acid,  methyl  ester,(Z,Z,Z)-­  (22.05%).  Three   major   compounds   were   identified   in   the   mixed   extract:   n-­hexadecanoic   acid  (16.58%),  phenol,2-­methoxy-­3-­(2-­propenyl)  (20.62%),  and  9,12,15-­ octadecatrienoic   acid,(Z,Z,Z)   (25.98%).   Four   of   the   compounds   in   the   mixed   extract   were   new:   eudesma-­4(14),11-­diene   (0.18%),   6-­ azabicyclo[3.2.1]octane   (0.51%),   cyclohexane,1-­ethyl-­1-­methyl-­2,4-­ bis(1-­methylenyl)-­,[1S-­␣␣␣␣␣␣␣␣␣␣␣-­␣-­Elemen   (0.77%),   and   globulol   (1.45%).   The   mixed   extract   had   a   high   level   of   antimicrobial   activity   against   fish   pathogens   as   indicated   by   zone   of   inhibition,   minimum   inhibitory  concentration,  and  minimum  bactericidal  concentration.
dc.format.extent 11 pages
dc.identifier.citation Harikrishnan, R., Kim, M., Kim, J., Balasundaram, C., Jawahar, S., & Heo, M. (2010). Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum . The Israeli Journal of Aquaculture - Bamidgeh, 62(2), 85-95.
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/19317
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh
dc.subject Azadirachta indica, Ocimum sanctum, antibacterial activity, bacteria
dc.subject.lcsh Fish culture--Israel--Periodicals.
dc.subject.lcsh Fish culture--Periodicals.
dc.subject.lcsh Aquaculture--Israel--Periodicals.
dc.subject.lcsh Aquaculture--Periodicals.
dc.title Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum
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