Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum

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dc.contributor.authorHarikrishnan, Ramasamy
dc.contributor.authorKim, Man-Chul
dc.contributor.authorKim, Ju-Sang
dc.contributor.authorBalasundaram, Chellam
dc.contributor.authorJawahar, Sundaram
dc.contributor.authorHeo, Moon-Soo
dc.date.accessioned2012-01-19T20:28:01Z
dc.date.available2012-01-19T20:28:01Z
dc.date.issued2010
dc.description.abstractThe  chemical  constituents  of  decoction  (individual)  and  concoction  (mixed)   of   ethanolic   leaf   extracts   from   Azadirachta   indica   (neem)   and   Ocimum   sanctum   (tulsi)   were   analyzed   by   gas   chromatography-­mass   spectro-­ photometery  (GC-­MS).  Decoctions  of  A.  indica  and  O.  sanctum  had  24  and   33   constituents,   respectively.   Mixed   together,   26   compounds   were   identified.   Four   major   (high   percentage)   compounds   were   identified   in   A.   indica:   n-­hexadecanoic   acid   (14.34%),   phytol   (19.96%),   9,12,15-­octa-­ decatrienoic   acid,   (Z,Z,Z)-­   (18.57%),   and   vitamin   E   (11.37%).   Three   major  compounds  were  identified  in  O.  sanctum:  phenol,2-­methoxy-­3-­(2-­ propenyl)   (15.32%),   9,12,15-­octadecatrienoic   acid,(Z,Z,Z)-­   (16.94%),   and  9,12,15-­octadecatrienoic  acid,  methyl  ester,(Z,Z,Z)-­  (22.05%).  Three   major   compounds   were   identified   in   the   mixed   extract:   n-­hexadecanoic   acid  (16.58%),  phenol,2-­methoxy-­3-­(2-­propenyl)  (20.62%),  and  9,12,15-­ octadecatrienoic   acid,(Z,Z,Z)   (25.98%).   Four   of   the   compounds   in   the   mixed   extract   were   new:   eudesma-­4(14),11-­diene   (0.18%),   6-­ azabicyclo[3.2.1]octane   (0.51%),   cyclohexane,1-­ethyl-­1-­methyl-­2,4-­ bis(1-­methylenyl)-­,[1S-­␣␣␣␣␣␣␣␣␣␣␣-­␣-­Elemen   (0.77%),   and   globulol   (1.45%).   The   mixed   extract   had   a   high   level   of   antimicrobial   activity   against   fish   pathogens   as   indicated   by   zone   of   inhibition,   minimum   inhibitory  concentration,  and  minimum  bactericidal  concentration.
dc.format.extent11 pages
dc.identifier.citationHarikrishnan, R., Kim, M., Kim, J., Balasundaram, C., Jawahar, S., & Heo, M. (2010). Identification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum . The Israeli Journal of Aquaculture - Bamidgeh, 62(2), 85-95.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19317
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectAzadirachta indica, Ocimum sanctum, antibacterial activity, bacteria
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleIdentification  and  Antimicrobial  Activity  of  Combined  Extract  from  Azadirachta  indica  and  Ocimum  sanctum

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IJA Volume 62, Issue 2, 2010