The effect of dietary docosahexaenoic acid (DHA; 22:6n-3) on photoreceptor abundance, rhodopsin expression and growth in developing gilthead sea bream (<em>Sparus aurata</em>) larvae
| dc.contributor.author | Tandler, Amos | |
| dc.contributor.author | Levitan, Anton | |
| dc.contributor.author | Nixon, Oriya | |
| dc.contributor.author | Gaon, Aviad | |
| dc.contributor.author | Koven, William | |
| dc.date.accessioned | 2023-06-07T19:43:35Z | |
| dc.date.available | 2023-06-07T19:43:35Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | This study determined the effect of prey DHA on larval gilthead sea bream (GSB; <em>Sparus aurata</em>) photoreceptor abundance, rhodopsin expression, and growth performance. It was carried out in a twenty-eight 400 l conical tank system that was stocked with 100 viable GSB eggs/l/tank. This allowed the testing of 4 levels of rotifer DHA; 0.99 (Low; L), 1.9 (Intermediate low; I-L), 3.2 (Intermediate high; I-H) and 12.1(High; H) mg DHA/g DW rotifer, which were fed (10 rotifers/ml) to 3-16 DPH larvae. These rotifer diets continued to be offered to 17-34 DPH fish, although these larvae predominantly fed on 4 DHA enriched Artemia nauplii treatments that were offered at a concentration from 0.1 nauplii/ml to 4 nauplii/ml, depending on larval age. This resulted in 4 DHA rotifer-Artemia ranges: 0.99-0.0 (L), 1.9-2.6 (I-L), 3.2-7.2 (I-H), and 12.1-11.77 (H) mg DHA/g DW. The 4 DHA treatments and ranges were tested in replicates of 7 conical tanks per treatment. Increasing rotifer DHA significantly (P<0.0001) improved TL, in an exponential manner, throughout larval rearing. DW in 34 DPH larvae was markedly (P<0.05) enhanced with dietary DHA inclusion in the rotifers and Artemia. There was a significant (P < 0.005) prey DHA dose dependent range effect on the abundance of photoreceptor cells in the retina of 34 DPH larvae. The gene expression of rhodopsin in GSB larvae was significantly (P<0.05) upregulated with dietary DHA dose range and larval age (P<0.0001). This study established a link between dietary DHA level with photoreceptor abundance and rhodopsin expression, which led to improved vision, prey acquisition, and growth in developing GSB larvae. | |
| dc.format.extent | 15 pages | |
| dc.identifier.doi | https://doi.org/10.46989/001c.77592 | |
| dc.identifier.issn | 0792-156X | |
| dc.identifier.uri | https://hdl.handle.net/10524/69696 | |
| dc.relation.ispartof | The Israeli Journal of Aquaculture - Bamidgeh | |
| dc.subject | docosahexaenoic acid | |
| dc.subject | fish larvae | |
| dc.subject | fish larval growth | |
| dc.subject | photoreceptor | |
| dc.subject | rhodopsin | |
| dc.subject | vision | |
| dc.title | The effect of dietary docosahexaenoic acid (DHA; 22:6n-3) on photoreceptor abundance, rhodopsin expression and growth in developing gilthead sea bream (<em>Sparus aurata</em>) larvae | |
| dc.type | Article | |
| dc.type.dcmi | Text | |
| prism.volume | 75 |
Files
Original bundle
1 - 1 of 1