New rRNA primers for the detection of Vibrio anguillarum
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2015
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Abstract
In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.
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Vibrio anguillarum, PCR, Fish culture--Israel., Fish culture.
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6 pages
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The Israeli Journal of Aquaculture - Bamidgeh
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