New rRNA primers for the detection of Vibrio anguillarum

Date
2015
Authors
Avsever, Meriç Lütfi
Tunalıgil, Serra
Didinen, Behire Işıl
Metin, Seçil
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.
Description
Keywords
Vibrio anguillarum, PCR, Fish culture--Israel., Fish culture.
Citation
Extent
6 pages
Format
Geographic Location
Time Period
Related To
The Israeli Journal of Aquaculture - Bamidgeh
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.