New rRNA primers for the detection of Vibrio anguillarum

dc.contributor.author	Avsever, Meriç Lütfi
dc.contributor.author	Tunalıgil, Serra
dc.contributor.author	Didinen, Behire Işıl
dc.contributor.author	Metin, Seçil
dc.date.accessioned	2016-05-05T22:58:17Z
dc.date.available	2016-05-05T22:58:17Z
dc.date.issued	2015
dc.description.abstract	In this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.
dc.format.extent	6 pages
dc.identifier.issn	0792-156X
dc.identifier.uri	http://hdl.handle.net/10524/49220
dc.relation.ispartof	The Israeli Journal of Aquaculture - Bamidgeh
dc.subject	Vibrio anguillarum
dc.subject	PCR
dc.subject.lcsh	Fish culture--Israel.
dc.subject.lcsh	Fish culture.
dc.title	New rRNA primers for the detection of Vibrio anguillarum
dc.type	Article
dc.type.dcmi	Text

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