New rRNA primers for the detection of Vibrio anguillarum

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dc.contributor.authorAvsever, Meriç Lütfi
dc.contributor.authorTunalıgil, Serra
dc.contributor.authorDidinen, Behire Işıl
dc.contributor.authorMetin, Seçil
dc.date.accessioned2016-05-05T22:58:17Z
dc.date.available2016-05-05T22:58:17Z
dc.date.issued2015
dc.description.abstractIn this work, 51 V. anguillarum isolates (42/51 of O1 serotype, 9/51 of O2) obtained from cultivated marine fish by Avsever and Ün (2015) were used. These isolates were confirmed with universal primers specific to amiB genes. They were also detected successfully at a rate of 100% with a new primer couple designed according to 16S ribosomal RNA sequence. The new primers were found to have a high sensitivity and specificity but were liable to give a cross-reaction with Vibrio ordalii.
dc.format.extent6 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/49220
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectVibrio anguillarum
dc.subjectPCR
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture.
dc.titleNew rRNA primers for the detection of Vibrio anguillarum
dc.typeArticle
dc.type.dcmiText

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Volume 67, 2015