Epidemiological Survey of Grass Carp (Ctenopharyngodon idella) Reovirus in South China, and Genetic Variations of VP6 gene
Date
2017
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
To study the molecular biology characteristics, phylogenetic relationship, and status quo of grass carp reovirus (GCRV) prevalent isolates, we conducted an epidemiological survey in South China from 2012 to 2016. We sampled 126 fish collected from five provinces. Eight strains of GCRV were isolated and examined in order to understand their genetic characteristics and evolution regularity. The results showed three genotypes of GCRV, of which. Genotype Ⅱ was found to be the prevalent strain in South China. SDS-PAGE electrophoresis further suggested that all viruses possess 11 segments of dsRNA, which have typical characteristics of GCRV. Based on amino acid sequence phylogenetic tree of VP6 gene, all eight isolates belonged to genotype Ⅱ and had significant variations compared to genotype Ⅰ. Analysis of amino acid sequence showed that these two genotypes of GCRV had no immunological cross-reactions. These results indicated that genotype Ⅱ GCRV is an extensive pandemic, and revealed new genetic diversity in China.
Description
Keywords
Grass carp reovirus, virus isolates, genetic variation, identification, immunogenicity, Fish culture--Israel., Fish culture
Citation
Extent
9 pages
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.